The BioMetal-Contaminants project will develop real time Laser Induced Breakdown spectroscopy (LIBS)-techniques and apply two existing vibrational techniques Surface-Enhanced Raman Spectroscopy (SERS)/FTIR chemical mapping with an ultimate goal to monitor the Bio-contamination in water and foods (E. coli and Salmonella enterica), and the metal contamination and nutritious elements in Agricultural Soils and also to improve the efficiency and detection time. The application of single pulse LIBS and development of dual-pulse LIBS (DPLIBS) and resonance-enhanced LIBS for decreasing the limit of detection and improving reproducibility of LIBS in heavy metal detection, agricultural soil/nutrients and bacterial contamination in Water and Foods are the new research approaches. The implementation of our findings will introduce new research approaches for developing better sensing spectroscopic techniques in food-water-soil industry and agriculture than are available today. The application of robust and practical vibrational spectroscopic techniques and signal enhancement methods will also, via the use of nanoparticles in the case of nanoparticle-enhanced (NELIBS) will lead to the development of different methods for improving the sensitivity and reproducibility. Metallic nanoparticles (NPs) and roughened metallic substrates have been used to enhance the Raman signal via surface plasmon resonance in SERS. The effect has been widely utilized in SERS measurements, not only does it increases the local density of the bacteria, but it also makes bacteria contact the SERS substrate. The type of bacteria could be differentiated in all cases studied along with the metabolic state (viable or heat killed). Water samples from industries and agricultural strata tend to have a lot of pollutants making it harmful for consumption. The determination of toxic metals by the high-sensitive techniques of DPLIBS and RELIBS will improve the limit of detection and also provide valuable information to those experiments performed by all partners.